Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese
نویسندگان
چکیده
Lactic acid bacteria (LAB) were isolated from ovine milk and cheeses manufactured in the South Region of Brazil. Among 112 bacterial isolates investigated, 59 were chosen through a screening for LAB. Among these 59 strains of LAB, 21% showed antimicrobial, proteolytic and lipolytic activities. Based on this screening, Lactobacillus plantarum LCN 17 and Lactobacillus rhamnosus LCN 43 were selected and tested for the production of bacteriocin-like substances (BLS). The BLS produced by both isolates showed antimicrobial activity against Listeria monocytogenes, whereas that produced by L. plantarum LCN 17 presented higher stability to different temperature, pH and enzyme treatments. These strains present potential for production of BLS, and for use as starter cultures.
منابع مشابه
A Study on the Antibacterial Activity of Lactic Acid Bacteria Isolated from Traditional Iranian Milk Samples
198 Lactic acid bacteria (LAB) were isolated from Iranian milk samples. Almost all isolatedLAB showed inhibitory activity towards E. coli and S. aureus as Gram negative and Grampositive indicators, respectively. No role was observed for hydrogen peroxide in the activity ofall isolated strains while organic acids proved a prominent role in all detected inhibitoryactivities. Within isolated LAB, ...
متن کاملIn Vitro Evaluation of Bacteriocin-Like Inhibitory Substances Produced by Lactic Acid Bacteria Isolated During Traditional Sicilian Cheese Making
Bacteriocins are antimicrobial proteins produced by bacteria that inhibit the growth of other bacteria with a bactericidal or bacteriostatic mode of action. Many lactic acid bacteria (LAB) produce a high diversity of different bacteriocins. Bacteriocinogenic LAB are generally recognised as safe (GRAS) and useful to control the frequent development of pathogens and spoilage microorganisms. For t...
متن کاملGROWTH INHIBITION OF PATHOGENS BY LACTIC ACID BACTERIA PRODUCING INHIBITORY SUBSTANCES
71 samples of milk and dairy products were collected from 18 cities throughout Iran. 93 strains of lactic acid bacteria (LAB) were isolated and assayed against four opportunistic patho gens including E. coli, proteus and klebsiella as gram-negative and S. aureus as a gram-positive organism. All indicators (organisms) were hospital strains. Among isolated bacteria, lactobacilli (37 isolates)...
متن کاملInhibitory Effect of Isolated Lactic Acid Bacteria from Scomberomorus commerson Intestines and their Bacteriocin on Listeria innocua
ABSTRACT- Lactic acid bacteria are commonly found in the gastrointestinal tract of various endothermic animals and humans, in milk, dairy and seafood products and on some plant surfaces. The aim of this study was to investigate the inhibitory effect of isolated lactic acid bacteria from intestines of narrow-barred Spanish mackerel (Scomberomorus commerson) on Listeria innocua growth. Five strai...
متن کاملIsolation of bacteriocin nisin producing Lactococcus lactis from dairy products
Most inhibitory substances produced by lactic acid bacteria are safe and effective natural inhibitors of pathogenic and food spoilage bacteria in various foods. In this study, bacteriocin nisin producing Lactococcus lactis was isolated from fermented milk and cheese. The test isolate was grown in MRS medium under optimized conditions. Bacteriocin activity of the test isolate was determined by a...
متن کامل